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Von Kossa silver staining indicated calcification providing evidence for the differentiation into the osteogenic lineage. We provide a marker panel for the unequivocal identification of spermatogonia providing a better basis for future studies on primate, including human, testis-derived stem cells. Published online Mar For the detection of intracellular antigens, the cells were permeabilized with 0. Alcian blue staining: detection of chondrocytes Chondrocytes are characterized by the production of proteoglycans, which can be detected with alcian blue. Interestingly, we propagated fibroblast-like cells that upon confluency formed colonies Fig. B The effect of bFGF on cell proliferation.

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